EveryLator
Log in
Sign up
Biology
Chemistry
Construction
Conversion
Ecology
Everyday life
Finance
Food
Health
Math
Physics
Sports
PCR Annealing Temperature Calculator
Forward Primer (5'→3')
0
bp,
0
% GC
Reverse Primer (5'→3')
0
bp,
0
% GC
PCR Product Length (bp)
Na⁺ (mM)
Mg²⁺ (mM)
Product %GC (Optional)
Leave blank to auto-estimate
Calculate Ta
Optimal Annealing Temperature
--
Range:
--
Primer Melting Temperatures
Forward Tm:
--
Reverse Tm:
--
Product Tm:
--
Primer Characteristics
Forward: Length / %GC:
--
Reverse: Length / %GC:
--
Temperature Range Visualization
55°C
65°C
Export CSV
Print
